UCMSC eXOrich medium is a serum-free and ready-to-use medium. The composition is optimized for the exosomes production from umbilical cord-derived mesenchymal stem cell (UCMSC). For exosomes production, we recommend that UCMSCs be cultivated in dishes or flasks until 90% confluence under regular culture conditions, and then remove the original culture medium and wash cells with the PBS and UCMSC eXOrich to remove the residual medium. Then the culture medium was replaced with UCMSC eXOrich and cultured for 3-4 days before collecting the conditioned medium for exosome purification.
      Users can also replace the original basal medium with UCMSC eXOrich, and subculture UCMSC for at least one passage, and then perform the above steps to obtain a conditioned medium for exosome purification.
      Using UCMSC eXOrich culture medium for exosome production, there is no need to add common nutritional supplements such as serum or human platelet lysate; however, users can decide to add appropriate nutritional supplements based on actual needs.

Exosome Harvest Workflow by eXOrich Medium: